4, cellulase concentration in the role of the environment

Cellulase enzymatic reaction chemical reactions on and the truth is the same, beginning with the role of the environment increases the concentration of cellulase, the reaction rate will gradually faster, but cellulase first and second in the reaction process Five to form an intermediate compound, further reaction, and therefore increases the cellulase, the cellulase and the contact area of ​​the substrate will be decreased, which leads to cellulase not smoothly play a role, and therefore the reaction environment if added too Multi-fiber enzyme, inhibit the cellulase their catalytic efficiency.

5, carbohydrates cellulase may play a protective role

The experiments show, sucrose, glucose, maltose and other sugars can cellulase may play a protective role. Add a protective agent method is an enzyme stabilization method most simple. At room temperature and high temperature have a very significant protective effect of trehalose on cellulose, glucose and sucrose at room temperature for cellulase has a protective effect, but at a high temperature will accelerate the inactivation of the enzyme. Glucan cellulase no protective effect at room temperature, but at high temperatures the enzyme showed a protective effect.

6, the impact of inhibitors and activators of cellulase

Cellulase enzymes are proteins, which in itself is an activity, and therefore, some of the heavy metal ions, trace elements and other substances will play an inhibitory effect cellulase activity, on the contrary some of the sodium ions will increase cellulase activity, in fiber luciferase course work should pay attention to the existence of the environment inhibitor or activator.

In short, not only the effect of cellulase strains and cellulases received quality of its own, but also by the influence of the environment, and therefore cellulases in industrial processes, good production process, a good method to use, even more important than the quality of their cellulase!